Article
IS30 activation of an smp'-lacZ gene fusion in Escherichia coli.
FEMS microbiology letters - 1 Mar 1990
Neuwald A F, Stauffer G V
Abstract excerpt
The Escherichia coli serB gene is divergently transcribed from the gene. Six independently isolated IS30 insertions within the 5' end of the serB structural gene resulted in increased expression of an smp'-lacZ gene fusion. DNA sequence analysis of two of the insertions suggests that a promoter was created upon IS30 insertion.
Topics
- Base Sequence
- Cloning, Molecular
- DNA Transposable Elements
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Lac Operon
- Molecular Sequence Data
- Mutation
- Phenotype
- Plasmids
- Promoter Regions, Genetic