Article
Mapping of insertion element IS30 in the Escherichia coli K12 chromosome.
Molecular & general genetics : MGG - 1 Jul 1990
Umeda M, Ohtsubo E
Abstract excerpt
We identified seven phage clones containing the insertion element IS30 in a lambda phage library mini-set, which includes 476 clones carrying chromosomal segments that cover almost the entire chromosome of Escherichia coli K12 W3110 (Kohara et al. 1987). We could assign locations and orientations to four copies of IS30 (named is30A to is30D) on the W3110 chromosome by restriction analysis of phage DNAs containing...
Topics
- Base Sequence
- Blotting, Southern
- Chromosome Mapping
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutation