Article
Heavy chain binding protein recognizes incompletely disulfide-bonded forms of vesicular stomatitis virus G protein.
The Journal of biological chemistry - 25 Apr 1990
Machamer C E, Doms R W, Bole D G, Helenius A, Rose J K
Abstract excerpt
To investigate the function of heavy chain binding protein (BiP, GRP 78) in the endoplasmic reticulum, we have characterized its interaction with a model plasma membrane glycoprotein, the G protein of vesicular stomatitis virus. We used a panel of well characterized mutant G proteins and immunoprecipitation with anti-BiP antibodies to determine if BiP interacted with newly synthesized G protein and/or mutant G...
Topics
- Animals
- Cell Line
- Chromosome Deletion
- Disulfides
- Endoplasmic Reticulum
- Glycosylation
- Macromolecular Substances
- Membrane Glycoproteins
- Mutation
- Protein Binding
- Transfection