Article
[Agrobacterium tumefaciens mediated transformation of Aspergillus niger conidiospores and sequence analysis of T-DNA insertion site in conidiation-defective mutant].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Feb 2011
Huang Wen, Yang Hongjiang, Qin Huibin
Abstract excerpt
OBJECTIVE: Development of a method of transforming Aspergillus niger conidiospores with Agrobacterium tumefaciens T-DNA system and using it as a tool for genome annotation via construction a T-DNA insertion mutant library of A. niger. METHODS: A. tumefaciens EHA105 carrying the binary vector pCAMBIA1301 was used to transform A. niger conidiospores under induction conditions. Hygromycin resistant transformants...
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