Article
Isolation of a Rhodobacter capsulatus mutant that lacks c-type cytochromes and excretes porphyrins.
Journal of bacteriology - 1 Mar 1990
Biel S W, Biel A J
Abstract excerpt
A Rhodobacter capsulatus mutant lacking cytochrome oxidase activity was isolated by Tn5 mutagenesis. Difference spectroscopy of crude extracts and extracted c-type cytochromes demonstrated that this mutant completely lacked all c-type cytochromes. The strain did, however, synthesize normal amounts of b-type cytochromes and nonheme iron. This mutant also excreted large amounts of coproporphyrin and protoporphyrin...
Topics
- Cloning, Molecular
- Cytochrome c Group
- Cytochromes
- DNA Transposable Elements
- Genes, Bacterial
- Mutation
- Porphyrins
- Restriction Mapping
- Rhodopseudomonas
