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Effect of Cytoplasmic Volume on Developmental Competence of Buffalo ( <i>Bubalus bubalis</i> ) Embryos Produced Through Hand-Made Cloning

12 May 2011

Abstract excerpt

This study examined the effects of cytoplasmic volume on the developmental competence of hand-made cloned buffalo embryos. Two different cell types, that is, buffalo fetal fibroblast (BFF) and buffalo embryonic stem (ES) cell-like cells were taken as donor cell and fused with one, two, or three demicytoplasts to generate embryos with decreased, normal (control), and increased cytoplasmic volume. Using BFF as a...

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PMID: 21563942
DOI: 10.1089/cell.2010.0096

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