Article
Circularity of the Caulobacter crescentus chromosome determined by pulsed-field gel electrophoresis.
Journal of bacteriology - 1 Mar 1990
Ely B, Ely T W, Gerardot C J, Dingwall A
Abstract excerpt
Previous genetic analyses of the Caulobacter crescentus chromosome have resulted in the construction of a linear genetic map. To establish the circularity of the C. crescentus chromosome, restriction fragments generated by digestion with AseI and SpeI were analyzed by pulsed-field gel electrophoresis and Southern hybridization. The size of each fragment was calculated and used to demonstrate that C. crescentus...
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