Bipolar localization of the replication origin regions of chromosomes in vegetative and sporulating cells of B. subtilis.

doi:10.1016/s0092-8674(00)81909-1

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Article

Bipolar localization of the replication origin regions of chromosomes in vegetative and sporulating cells of B. subtilis.

Cell - 7 Mar 1997

Webb C D, Teleman A, Gordon S, Straight A, Belmont A, Lin D C, Grossman A D, Wright A, Losick R

Abstract excerpt

To investigate chromosome segregation in B. subtilis, we introduced tandem copies of the lactose operon operator into the chromosome near the replication origin or terminus. We then visualized the position of the operator cassettes with green fluorescent protein fused to the Lac1 repressor. In sp...

Topics

Bacillus subtilis
Cell Division
Chromosomes, Bacterial
Mutation
Polyploidy
Replication Origin
Spores, Bacterial

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