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Self-Renewing Human Bone Marrow Mesenspheres Promote Hematopoietic Stem Cell Expansion

25 Apr 2013

Abstract excerpt

Strategies for expanding hematopoietic stem cells (HSCs) include coculture with cells that recapitulate their natural microenvironment, such as bone marrow stromal stem/progenitor cells (BMSCs). Plastic-adherent BMSCs may be insufficient to preserve primitive HSCs. Here, we describe a method of isolating and culturing human BMSCs as nonadherent mesenchymal spheres. Human mesenspheres were derived from CD45- CD31-...

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PMID: 23623496
DOI: 10.1016/j.celrep.2013.03.041

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