Article
Genetic variability of Chlamydophila abortus strains assessed by PCR-RFLP analysis of polymorphic membrane protein-encoding genes.
Veterinary microbiology - 5 Aug 2011
Sait Michelle, Clark Ewan M, Wheelhouse Nicholas, Spalding Lucy, Livingstone Morag, Sachse Konrad, Markey Bryan K, Magnino Simone, Siarkou Victoria I, Vretou Evangelia, Caro María R, Yaga Raja, Lainson F Alex, Smith David G E, Wright Frank, Longbottom David
Abstract excerpt
This study used PCR-RFLP to investigate the genetic variability of pmp-encoding genes from fifty-two Chlamydophila abortus (C. abortus) strains originating from abortion cases from various geographical regions and host species. Six primer pairs were used to PCR-amplify DNA fragments encoding eighteen pmps. PCR products were digested using four restriction endonucleases and Bayesian methodologies were used to...
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