Article
Characterization of TrdL as a 10-hydroxy dehydrogenase and generation of new analogues from a tirandamycin biosynthetic pathway.
Organic letters - 6 May 2011
Mo Xuhua, Huang Hongbo, Ma Junying, Wang Zhongwen, Wang Bo, Zhang Si, Zhang Changsheng, Ju Jianhua
Abstract excerpt
TrdL, encoding a flavin-dependent oxidoreductase in the tirandamycin gene cluster, was inactivated to afford a ΔtrdL mutant, the fermentation of which yielded a new intermediate, tirandamycin E (5), and an additional early intermediate, tirandamycin F (6), if XAD-16 resin was introduced. TrdL was overexpressed in E. coli, and the protein was shown to efficiently catalyze the transformations from 5 to tirandamycin...
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