Article
Intragenic suppression of a capsid assembly-defective P22 tailspike mutation.
Genetics - 1 Aug 1990
Maurides P A, Schwarz J J, Berget P B
Abstract excerpt
The tailspike protein of bacteriophage P22 assembles with mature capsids during the final reaction in phage morphogenesis. The gene 9 mutation hmH3034 synthesizes a tailspike protein with a change at amino acid 100 from Asp to Asn. This mutant form of trimeric tailspike protein fails to assemble with capsids in vivo. By using in vitro quantitative tailspike-capsid assembly assays, this mutant tailspike trimer can...
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