Article
A PCR-based strategy for Dombrock screening in Brazilian blood donors reveals a novel allele: the DO* A-WL.
Journal of clinical laboratory analysis - 1 Jan 2011
Baleotti Wilson, Suzuki Rodrigo Buzinaro, Polotto Milena, Ruiz Marcelo Ortega, Fabron Antonio, Castilho Lilian
Abstract excerpt
BACKGROUND: Determination of the molecular basis underlying the antigens in the Dombrock blood group system has shown various rearrangements between the alleles associated with DO(*) A and DO(*) B. Based on this, we employed a PCR-based strategy to screen DO alleles (DO(*) A, DO(*) B, HY(*) 1, HY(*) 2 and JO) in Brazilians. METHODS: We tested DNA of 278 Brazilian blood donors by PCR-RFLP on plates of 96 wells to...
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