Article
The essential carboxyl group in subunit c of the F1F0 ATP synthase can be moved and H(+)-translocating function retained.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jul 1990
Miller M J, Oldenburg M, Fillingame R H
Abstract excerpt
The proteolipid subunit c of F1F0-type H(+)-transporting ATP synthases [ATP phosphohydrolase (H(+)-transporting), EC 3.6.1.34] contains a conserved Asp/Glu residue that is thought to function in H+ translocation. To test the importance of the position of this residue in the Escherichia coli enzyme, we used oligonucleotide-directed mutagenesis to move the carboxyl side chain from position 61 to position 58, 60, or...
Topics
- Amino Acid Sequence
- Base Sequence
- Cell Membrane
- Chromosomes, Bacterial
- Escherichia coli
- Genes, Bacterial
- Hydrogen-Ion Concentration
- Kinetics
- Macromolecular Substances
- Molecular Sequence Data