Article
Suppressor mutations in F1 subunit epsilon recouple ATP-driven H+ translocation in uncoupled Q42E subunit c mutant of Escherichia coli F1F0 ATP synthase.
The Journal of biological chemistry - 8 Apr 1994
Zhang Y, Oldenburg M, Fillingame R H
Abstract excerpt
The Q42E mutation in the polar loop of subunit c of the Escherichia coli F1F0 ATP synthase leads to an uncoupling of H+ translocation through F0 and ATP synthesis/hydrolysis in F1. We have isolated four second-site suppressor mutants in which the coupling defect is corrected. Substitutions for Glu31 in F1 subunit epsilon were found in each suppressor mutant, where the substitutions were E31G, E31V, and E31K (the...
Topics
- Adenosine Triphosphate
- Biological Transport
- Cell Membrane
- Escherichia coli
- Genes, Suppressor
- Glutamates
- Glutamic Acid
- Hydrogen
- Mutation
- Operon
- Proton-Translocating ATPases