Article
Agrobacterium rhizogenes-mediated transformation of Prunus as an alternative for gene functional analysis in hairy-roots and composite plants.
Plant cell reports - 1 Jul 2011
Bosselut Nathalie, Van Ghelder Cyril, Claverie Michel, Voisin Roger, Onesto Jean-Paul, Rosso Marie-Noëlle, Esmenjaud Daniel
Abstract excerpt
Resistant rootstocks offer an alternative to pesticides for the control of soil pests. In Prunus spp., resistance loci to root-knot nematodes (RKN) have been mapped and a transformation method is needed to validate candidate genes. Our efforts have focused on the generation of transformed hairy-roots and composite plants appropriate for nematode infection assays. An efficient and reliable method using the A4R...
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