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A multiplex one-step real-time RT-PCR assay for influenza surveillance

17 Feb 2011

Abstract excerpt

For surveillance purposes real-time PCR assays for influenza viruses had to be adapted to the pandemic influenza A(H1N1)2009 strain. We combined published primers and probes for influenza A, influenza B and an internal amplification control with a detection system for influenza A(H1N1)2009 to set up a rapid, reliable, simple and cost-effective high-throughput multiplex one-step real-time RT-PCR. The workflow also...

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PMID: 21345319
DOI: 10.2807/ese.16.07.19798-en

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