Article
Single-Step Multiplex Reverse Transcription–Polymerase Chain Reaction (RT-PCR) for Influenza A Virus Subtype H5N1 Detection
1 Dec 2004
Abstract excerpt
Influenza A virus subtype H5N1 causes a rapidly fatal systemic disease in domestic poultry and spreads directly from poultry to humans. The aim of this study was to develop a rapid, cost-saving and effective method for influenza A virus subtype H5N1 detection. The selected primer set was used in single-step RT-PCR for simultaneous detection in multiplex format of the 276-, 189-, and 131-bp fragments,...
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