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Article

Cloning and expression of envelope protein of Thai genotype I strain KE-093 of Japanese encephalitis virus.

The Southeast Asian journal of tropical medicine and public health - 1 Nov 2010

Witthajitsomboon Nisakorn, Chen Antony, Lorroengsil Sasithorn, Sällberg Matti, Pantuwatana Somsak

Abstract excerpt

The purpose of this study was to clone and express envelope (E) gene of Japanese encephalitis virus (JEV) genotype I, Thai strain KE-093. The E gene was amplified by PCR and cloned using the expression vector, pET-15b. Analysis of the insert sequence revealed a point mutation, which was corrected by site directed mutagenesis. The envelope 53 kDa protein expression was generated by in vitro coupled transcription...

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PMID: 21329311

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