Article
Metabolic engineering to enhance bacterial hydrogen production.
Microbial biotechnology - 1 Jan 2008
Maeda Toshinari, Sanchez-Torres Viviana, Wood Thomas K
Abstract excerpt
Hydrogen fuel is renewable, efficient and clean, and fermentative bacteria hold great promise for its generation. Here we use the isogenic Escherichia coli K-12 KEIO library to rapidly construct multiple, precise deletions in the E. coli genome to direct the metabolic flux towards hydrogen production. Escherichia coli has three active hydrogenases, and the genes involved in the regulation of the formate hydrogen...
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