Article
Three-dimensional structures of aspartate aminotransferase from Escherichia coli and its mutant enzyme at 2.5 A resolution.
Journal of biochemistry - 1 Aug 1990
Kamitori S, Okamoto A, Hirotsu K, Higuchi T, Kuramitsu S, Kagamiyama H, Matsuura Y, Katsube Y
Abstract excerpt
The structure of Escherichia coli aspartate aminotransferase complex with the inhibitor 2-methylaspartate, and that of the mutant enzyme in which an arginine was substituted for a lysine residue thereby forming a Schiff base with the coenzyme pyridoxal 5'-phosphate, were determined at 2.5 A resol...
Topics
- Amino Acids
- Animals
- Arginine
- Aspartate Aminotransferases
- Chemical Phenomena
- Chemistry, Physical
- Crystallography
- Escherichia coli
- Kinetics
- Lysine
- Mutation
- Protein Conformation
- Schiff Bases
- Swine
- X-Ray Diffraction