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Real-time PCR-based quantification of Toxoplasma gondii in tissue samples of serologically positive outdoor chickens.

Memorias do Instituto Oswaldo Cruz - 1 Nov 2010

Aigner Cleiton Paulo, Silva Aristeu Vieira da, Sandrini Fabiano, Osório Paulo de Sá, Poiares Lilian, Largura Alvaro

Abstract excerpt

This study aimed to quantify Toxoplasma gondii in tissue samples of serologically positive chickens using real-time polymerase chain reaction (PCR). Of 65 chickens evaluated, 28 were positive for T. gondii antibodies. Brain and heart samples were collected from 26 seropositive chickens and DNA was extracted using Trizol® and amplified using real-time PCR with SYBR® Green. Parasite DNA was detected in 24 of the 26...

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PMID: 21120368
DOI: 10.1590/s0074-02762010000700018

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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