Characterization of PBSX, a defective prophage of Bacillus subtilis.

doi:10.1128/jb.172.5.2667-2674.1990

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Article

Characterization of PBSX, a defective prophage of Bacillus subtilis.

Journal of bacteriology - 1 May 1990

Wood H E, Dawson M T, Devine K M, McConnell D J

Abstract excerpt

PBSX, a defective Bacillus subtilis prophage, maps to the metA-metC region of the chromosome. DNA (33 kilobases) from this region of the chromosome was cloned and analyzed by insertional mutagenesis with the integrating plasmid pWD3. This plasmid had a promoterless alpha-amylase gene (amyL) that...

Topics

Bacillus subtilis
Bacteriophages
Chromosome Mapping
Chromosomes, Bacterial
Cloning, Molecular
Defective Viruses
Escherichia coli
Genes
Mutation
Plasmids
Promoter Regions, Genetic
Restriction Mapping
Transduction, Genetic
Transformation, Bacterial
alpha-Amylases

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