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Article

Purification and characterization of factor VIII 372-Cys: a hypofunctional cofactor from a patient with moderately severe hemophilia A.

Blood - 15 Apr 1990

O'Brien D P, Pattinson J K, Tuddenham E G

Abstract excerpt

We have purified factor VIII from a patient with moderately severe hemophilia A (FVIII, 4 U/dL; FVIII:Ag, 110 U/dL) and subjected the protein to Western blot analysis after time course activation with thrombin. The cross reacting material-positive (CRM+) FVIII has the normal distribution of heavy and light chains before thrombin activation, and, after incubation with the enzyme, appropriate cleavages are made at...

Topics

Amino Acid Sequence
Arginine
Blotting, Western
Codon
Cysteine
DNA
Factor VIII
Hemophilia A
Humans
Molecular Sequence Data
Mutation
Nucleic Acid Hybridization

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasma

Thrombin

von Willebrand Factor

Source

Identifiers

PMID: 2109644

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2109644-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Purification and characterization of factor VIII 372-Cys: a hypofunctional cofactor from a patient with moderately severe hemophilia A.PMID 2109644Read ↗

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