Article
Improvement in ACE I/D polymorphism detection.
Journal of the renin-angiotensin-aldosterone system : JRAAS - 1 Sept 2011
Bolli Paola, Sticchi Elena, Giusti Betti, Saracini Claudia, Abbate Rosanna, Fatini Cinzia
Abstract excerpt
The ACE I/D polymorphism has been reported to influence predisposition to cardiovascular disease. Conflicting results in its detection may be due to mistyping of I/D genotypes as D/D genotypes occurring in the traditional genotyping method. In order to resolve mistyping troubles and to permit a rapid and accurate analysis, we performed a stepdown PCR reaction followed by detection using Nanogen technology, and we...
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