Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Quantitative Detection of Residual E. coli Host Cell DNA by Real-Time PCR

28 Oct 2010

Abstract excerpt

E. coli has been widely used as a host system to manufacture recombinant proteins for human therapeutic use. Among impurities to be eliminated during the downstream process, residual host cell DNA is a major interest for safety. Residual E. coli host cell DNA in the final products are usually determined using conventional slot blot hybridization assay or total DNA Threshold assay, although these methods are time...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacteriophages and microbial interactions
Share to Molecular Biology Techniques and Applications
Share to Viral gastroenteritis research and epidemiology

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Source

Identifiers

PMID: 21030834
DOI: 10.4014/jmb.1004.04035

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 10 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 8 closest

Quantitative Detection of Residual E. coli Host Cell DNA by Real-Time PCRPMID 21030834Read ↗

A fusion of Taq DNA polymerase with the CL7 protein from Escherichia coli remarkably improves DNA amplificationSemantic proximity Read ↗

Development of droplet digital PCR-based detection of bacterial pathogens in prosthetic joint infection: a preliminary study using a synthesized model plasmidSemantic proximity Read ↗

An event-specific real-time PCR detection system for the transgenic rice line 114-7-2 of producing functional human serum albuminSemantic proximity Read ↗

Real-time recombinase-aided amplification with improved propidium monoazide for the rapid detection of viable Escherichia coli O157:H7 in milkSemantic proximity Read ↗

Establishment of a Method for Describing stx Genes Expression of E. coli O157:H7 in Ground Beef Matrix during Refrigerated StorageSemantic proximity Read ↗

Statistical tools for transgene copy number estimation based on real-time PCRSemantic proximity Read ↗

Modeling of 5′ Nuclease Real-Time Responses for Optimization of a High-Throughput Enrichment PCR Procedure for Salmonella entericaSemantic proximity Read ↗

The PCR Revolution: Basic Technologies and ApplicationsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.