Article
Phenotypic analysis using very small volumes of blood.
Current protocols in cytometry - 1 Oct 2010
Weaver James L, McKinnon Katherine, Germolec Dori R
Abstract excerpt
Analysis of cell-surface phenotype of peripheral blood leukocytes is one of the most common applications of flow cytometry. In mouse research, the small size of the animal limits the amount of blood available. Standard staining methods using lysis of erythrocytes or gradient separation followed by repeated washing involve unavoidable losses of cells that generally limit analysis of blood to terminal methods....
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