Article
The membrane subunit NuoL(ND5) is involved in the indirect proton pumping mechanism of Escherichia coli complex I.
The Journal of biological chemistry - 10 Dec 2010
Nakamaru-Ogiso Eiko, Kao Mou-Chieh, Chen Han, Sinha Subhash C, Yagi Takao, Ohnishi Tomoko
Abstract excerpt
Complex I pumps protons across the membrane by using downhill redox energy. Here, to investigate the proton pumping mechanism by complex I, we focused on the largest transmembrane subunit NuoL (Escherichia coli ND5 homolog). NuoL/ND5 is believed to have H(+) translocation site(s), because of a high sequence similarity to multi-subunit Na(+)/H(+) antiporters. We mutated thirteen highly conserved residues between...
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