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Article

Enhancement of the latent 3-isopropylmalate dehydrogenase activity of promiscuous homoisocitrate dehydrogenase by directed evolution.

The Biochemical journal - 1 Nov 2010

Suzuki Yumewo, Asada Kuniko, Miyazaki Junichi, Tomita Takeo, Kuzuyama Tomohisa, Nishiyama Makoto

Abstract excerpt

HICDH (homoisocitrate dehydrogenase), which is involved in lysine biosynthesis through α-aminoadipate, is a paralogue of IPMDH [3-IPM (3-isopropylmalate) dehydrogenase], which is involved in leucine biosynthesis. TtHICDH (Thermus thermophilus HICDH) can recognize isocitrate, as well as homoisocitrate, as the substrate, and also shows IPMDH activity, although at a considerably decreased rate. In the present study,...

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PMID: 20735360
DOI: 10.1042/BJ20101246

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