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Article

Functional analysis of human BRCA2 variants using a mouse embryonic stem cell-based assay.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2010

Kuznetsov Sergey G, Chang Suhwan, Sharan Shyam K

Abstract excerpt

We describe here a comprehensive and reliable assay to test the functional significance of variants of unknown clinical significance (VUS) identified in the human breast cancer susceptibility gene, BRCA2. The assay is based on the ability of human BRCA2 to complement the loss of endogenous Brca2 in mouse embryonic stem cells. The procedure involves generation of a desired mutation in BRCA2 present in a bacterial...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 20721749
DOI: 10.1007/978-1-60761-759-4_16
PMCID: PMC6669890

Genes

BRCA2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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