Article
Restoration of growth phenotypes of Escherichia coli DH5alpha in minimal media through reversal of a point mutation in purB.
Applied and environmental microbiology - 1 Sept 2010
Jung Suk-Chae, Smith Chris L, Lee Ki-Sung, Hong Min-Eui, Kweon Dae-Hyuk, Stephanopoulos Gregory, Jin Yong-Su
Abstract excerpt
A point mutation (E115K) resulting in slower growth of Escherichia coli DH5alpha and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.
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