Article
Molecular cloning and sequencing of a gene from alkaliphilic Bacillus firmus OF4 that functionally complements an Escherichia coli strain carrying a deletion in the nhaA Na+/H+ antiporter gene.
The Journal of biological chemistry - 5 Dec 1991
Ivey D M, Guffanti A A, Bossewitch J S, Padan E, Krulwich T A
Abstract excerpt
A gene has been cloned from a DNA library from alkaliphilic Bacillus firmus OF4 that functionally complements a mutant strain of Escherichia coli, NM81, that carries a deletion for one of that strain's Na+/H+ antiporter genes (delta nhaA). The cloned alkaliphile gene restored to NM81 the ability...
Topics
- Amino Acid Sequence
- Bacillus
- Base Sequence
- Blotting, Southern
- Carrier Proteins
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Humans
- Hydrogen-Ion Concentration
- Molecular Sequence Data
- Mutation