Article
Dried reagents for multiplex genotyping by tag-array minisequencing to be used in microfluidic devices.
The Analyst - 1 Sept 2010
Ahlford Annika, Kjeldsen Bastian, Reimers Jakob, Lundmark Anders, Romani Massimo, Wolff Anders, Syvänen Ann-Christine, Brivio Monica
Abstract excerpt
We present an optimized procedure for freeze-drying and storing reagents for multiplex PCR followed by genotyping using a tag-array minisequencing assay with four color fluorescence detection which is suitable for microfluidic assay formats. A test panel was established for five cancer mutations in three codons (175, 248 and 273) of the tumor protein gene (TP53) and for 13 common single nucleotide polymorphisms...
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