Article
A specific DNA sequence is required for high frequency of recombination in the ade6 gene of fission yeast.
The EMBO journal - 1 Aug 1991
Schuchert P, Langsford M, Käslin E, Kohli J
Abstract excerpt
The point mutation M26 in the ade6 gene of Schizosaccharomyces pombe increases recombination frequency by an order of magnitude in comparison with other mutations in the same gene. The hypothesis is tested that this hot spot of recombination requires a specific nucleotide sequence at the M26 site. The DNA sequence is altered systematically by in vitro mutagenesis, and the resulting sequences are introduced into...
Topics
- Alleles
- Base Sequence
- DNA, Fungal
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Fungal
- Genes, Bacterial
- Genes, Fungal
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Recombination, Genetic