Article
Crystallization and preliminary X-ray diffraction analysis of the Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant.
Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Jul 2010
Protsko Carla, Vieille Claire, Laivenieks Maris, Prasad Lata, Sanders David A R, Delbaere Louis T J
Abstract excerpt
The Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant is stereospecific for (R)-alcohols instead of (S)-alcohols. Pyramidal crystals grown in the presence of (R)-phenylethanol via the hanging-drop vapour-diffusion method diffracted to 3.2 A resolution at the Canadian Light Source. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 80.23, b =...
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