Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimization and analysis of a quantitative real-time PCR-based technique to determine microRNA expression in formalin-fixed paraffin-embedded samples

23 Jun 2010

Abstract excerpt

BACKGROUND: MicroRNAs (miRs) are non-coding RNA molecules involved in post-transcriptional regulation, with diverse functions in tissue development, differentiation, cell proliferation and apoptosis. miRs may be less prone to degradation during formalin fixation, facilitating miR expression studies in formalin-fixed paraffin-embedded (FFPE) tissue. RESULTS: Our study demonstrates that the TaqMan Human MicroRNA...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to MicroRNA in disease regulation
Share to Molecular Biology Techniques and Applications
Share to Osteoarthritis Treatment and Mechanisms

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 20573258
DOI: 10.1186/1472-6750-10-47

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimization and analysis of a quantitative real-time PCR-based technique to determine microRNA expression in formalin-fixed paraffin-embedded samplesPMID 20573258Read ↗

Improved RNA quality and TaqMan®Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materialsSemantic proximity Read ↗

Validation of a microRNA-based qRT-PCR test for accurate identification of tumor tissue originSemantic proximity Read ↗

RNAscope in situ hybridization confirms mRNA integrity in formalin-fixed, paraffin-embedded cancer tissue samplesSemantic proximity Read ↗

Influence of formalin fixation duration on RNA quality and quantity from formalin‐fixed paraffin‐embedded hepatocellular carcinoma tissuesSemantic proximity Read ↗

Micromarkers 2.0: an update on the role of microRNAs in cancer diagnosis and prognosis.Semantic proximity Read ↗

Correlation of miRNA Expression Profiling in Surgical Pathology Materials, with Ki-67, HER2, ER and PR in Breast Cancer PatientsSemantic proximity Read ↗

Comparison of Two Illumina Whole Transcriptome RNA Sequencing Library Preparation Methods Using Human FFPE SpecimensSemantic proximity Read ↗

Mining the archives: a cross-platform analysis of gene expression profiles in archival formalin-fixed paraffin-embedded (FFPE) tissueSemantic proximity Read ↗

Quality control recommendations for RNASeq using FFPE samples based on pre-sequencing lab metrics and post-sequencing bioinformatics metricsSemantic proximity Read ↗

Automated extraction of DNA and RNA from a single formalin-fixed paraffin-embedded tissue section for analysis of both single-nucleotide polymorphisms and mRNA expression.Semantic proximity Read ↗

A novel method for RNA extraction from FFPE samples reveals significant differences in biomarker expression between orthotopic and subcutaneous pancreatic cancer patient-derived xenograftsSemantic proximity Read ↗

Impact of RNA Extraction and Target Capture Methods on RNA Sequencing Using Formalin-Fixed, Paraffin Embedded TissuesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.