Major histocompatibility complex independent T cell receptor-antigen interaction: functional analysis using fluorescein derivatives.

doi:10.1084/jem.174.1.229

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Article

Major histocompatibility complex independent T cell receptor-antigen interaction: functional analysis using fluorescein derivatives.

The Journal of experimental medicine - 1 Jul 1991

Diamond D J, Szalay P, Symer D, Hao P, Shin H S, Dintzis R Z, Dintzis H M, Reinherz E L, Siliciano R F

Abstract excerpt

We have isolated T cell receptor (TCR) cDNAs from fluorescein (FL)-specific human T cell clones (alpha FL beta FL), and transferred them to TCR beta- Jurkat cells in order to study direct FL-binding to the TCR. Using either FL-conjugated polymers (FL-polymer) or FL-substituted Sepharose beads, we are able to demonstrate the direct binding of antigen to the T cell surface, and the functional activation of the T...

Topics

Amino Acid Sequence
Cell Line
Cloning, Molecular
Flow Cytometry
Fluoresceins
Genetic Variation
Genetic Vectors
Humans
Macromolecular Substances
Major Histocompatibility Complex

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Receptors, Antigen, T-Cell

Restriction Mapping

Sepharose

T-Lymphocytes

Transfection

Source

Open in PubMed Open full text

Identifiers

PMID: 2056277
DOI: 10.1084/jem.174.1.229
PMCID: PMC2118876

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2056277-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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