Article
Analysis of the diffusion of Ras2 in Saccharomyces cerevisiae using fluorescence recovery after photobleaching.
Physical biology - 4 Jun 2010
Vinnakota Kalyan C, Mitchell David A, Deschenes Robert J, Wakatsuki Tetsuro, Beard Daniel A
Abstract excerpt
Binding, lateral diffusion and exchange are fundamental dynamic processes involved in protein association with cellular membranes. In this study, we developed numerical simulations of lateral diffusion and exchange of fluorophores in membranes with arbitrary bleach geometry and exchange of the membrane-localized fluorophore with the cytosol during fluorescence recovery after photobleaching (FRAP) experiments. The...
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