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Evaluation and Attempted Optimization of Avian Embryos and Cell Culture Methods for Efficient Isolation and Propagation of Low Pathogenicity Avian Influenza Viruses

1 Mar 2010

Abstract excerpt

Surveillance of wild bird populations for avian influenza viruses (AIV) contributes to our understanding of AIV evolution and ecology. Both real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) and virus isolation in embryonating chicken eggs (ECE) are standard methods for detecting AIV in swab samples from wild birds, but AIV detection rates are higher with RRT-PCR than isolation in ECE. In this...

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PMID: 20521704
DOI: 10.1637/8837-040309-reg.1

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