Article
Rapid and sensitive allele-specific (AS)-RT-PCR assay for detection of T315I mutation in chronic myeloid leukemia patients treated with tyrosine-kinase inhibitors.
Clinical and experimental medicine - 1 Mar 2011
Manrique Arechavaleta Gonzalo, Scholl Vanesa, Pérez Verónica, Bittencourt Roberta, Moellmann Arthur, Hassan Rocio, Seuánez Héctor N, Dobbin Jane, Martinez Lem, Renault Ilana Zalcberg, Uriarte Rosario
Abstract excerpt
Point mutations in the kinase domain of BCR-ABL were described in 40-90% of patients with chronic myeloid leukemia (CML) resistant to Imatinib. We herein describe the development of a rapid allele-specific (AS)-RT-PCR assay to identify the T315I mutation, which confers full resistance to all available tyrosine-kinase inhibitors (TKI). The mutation status of 65 patients with resistant CML was evaluated, and the...
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