Article
Sequence heterogeneity in the equi merozoite antigen gene (ema-1) of Theileria equi and development of an ema-1-specific TaqMan MGB assay for the detection of T. equi.
Veterinary parasitology - 27 Aug 2010
Bhoora Raksha, Quan Melvyn, Matjila Paul T, Zweygarth Erich, Guthrie Alan J, Collins Nicola E
Abstract excerpt
Although a quantitative real-time PCR assay (qPCR) assay for the detection of Theileria equi has been developed and evaluated, it is possible that additional, as yet undetected 18S rRNA gene sequence variants may exist. A qPCR assay targeting a different gene, used in conjunction with the T. equi 18S rRNA qPCR assay, could assist in the detection of all T. equi genotypes in field samples. A T. equi ema-1-specific...
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