Article
Development and evaluation of real-time PCR assays for the quantitative detection of Babesia caballi and Theileria equi infections in horses from South Africa.
Veterinary parasitology - 25 Mar 2010
Bhoora Raksha, Quan Melvyn, Franssen Linda, Butler Catherine M, van der Kolk Johannes H, Guthrie Alan J, Zweygarth Erich, Jongejan Frans, Collins Nicola E
Abstract excerpt
A quantitative real-time polymerase chain reaction (qPCR) assay using a TaqMan minor groove binder (MGB) probe was developed for the detection of Babesia caballi infection in equids from South Africa. Nine previously published sequences of the V4 hypervariable region of the B. caballi 18S rRNA gene were used to design primers and probes to target unique, conserved regions. The B. caballi TaqMan MGB qPCR assay was...
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