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Article

[Development of 3-enzyme pyrosequencing system and its application in rapid diagnosis of Down's syndrome].

Yi chuan = Hereditas - 1 May 2010

Liu Xi-Qun, Zhu Shu-Hui, Zou Bing-Jie, Ma Yin-Jiao, Zhou Guo-Hua

Abstract excerpt

To avoid sequencing error resulting from use of apyrase in conventional 4- enzyme pyrosequencing system, a non-apyrase 3-enzyme pyrosequencing system with a better performance of quantitative analysis was established. The method is to immobilize biotinylated DNA template, ATP sulfurylase and luci...

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Identifiers

PMID: 20466643
DOI: 10.3724/sp.j.1005.2010.00517

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-20466643-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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