Article
Requirements for transitional endoplasmic reticulum site structure and function in Saccharomyces cerevisiae.
Molecular biology of the cell - 1 May 2010
Shindiapina Polina, Barlowe Charles
Abstract excerpt
Secretory proteins are exported from the endoplasmic reticulum (ER) at specialized regions known as the transitional ER (tER). Coat protein complex II (COPII) proteins are enriched at tER sites, although the mechanisms underlying tER site assembly and maintenance are not understood. Here, we investigated the dynamic properties of tER sites in Saccharomyces cerevisiae and probed protein and lipid requirements for...
Topics
- COP-Coated Vesicles
- Cerulenin
- Cycloheximide
- Endoplasmic Reticulum
- Fatty Acid Synthesis Inhibitors
- Fatty Acids
- GTPase-Activating Proteins
- Green Fluorescent Proteins
- Guanine Nucleotide Exchange Factors
- Immunoblotting
- Membrane Glycoproteins
- Membrane Proteins
- Microscopy, Confocal