Article
Evidence that histidine forms a coordination bond to the A(0A) and A(0B) chlorophylls and a second H-bond to the A(1A) and A(1B) phylloquinones in M688H(PsaA) and M668H(PsaB) variants of Synechocystis sp. PCC 6803.
Biochimica et biophysica acta - 1 Aug 2014
Sun Junlei, Hao Sijie, Radle Matthew, Xu Wu, Shelaev Ivan, Nadtochenko Victor, Shuvalov Vladimir, Semenov Alexey, Gordon Heather, van der Est Art, Golbeck John H
Abstract excerpt
The axial ligands of the acceptor chlorophylls, A(0A) and A(0B), in Photosystem I are the Met sulfur atoms of M688(PsaA) and M668(PsaB). To determine the role of the Met, His variants were generated in Synechocystis sp. PCC 6803. Molecular dynamics simulations on M688H(PsaA) show that there exist low energy conformations with the His coordinated to A(0A) and possibly H-bonded to A(1A). Transient EPR studies on...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.