Article
Presence of a [3Fe-4S] cluster in a PsaC variant as a functional component of the photosystem I electron transfer chain in Synechococcus sp. PCC 7002.
Photosynthesis research - 1 Apr 2018
Pérez Adam A, Ferlez Bryan H, Applegate Amanda M, Walters Karim, He Zhihui, Shen Gaozhong, Golbeck John H, Bryant Donald A
Abstract excerpt
A site-directed C14G mutation was introduced into the stromal PsaC subunit of Synechococcus sp. strain PCC 7002 in vivo in order to introduce an exchangeable coordination site into the terminal FB [4Fe-4S] cluster of Photosystem I (PSI). Using an engineered PSI-less strain (psaAB deletion), psaC was deleted and replaced with recombinant versions controlled by a strong promoter, and the psaAB deletion was...
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