Article
Nested Patch PCR for highly multiplexed amplification of genomic loci.
Cold Spring Harbor protocols - 1 Jul 2009
Varley Katherine E, Mitra Robi D
Abstract excerpt
Nested Patch polymerase chain reaction (PCR) amplifies a large number (greater than 90) of targeted loci from genomic DNA simultaneously in the same reaction. These amplified loci can then be sequenced on a second-generation sequencing machine to detect single nucleotide polymorphisms (SNPs) and...
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