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Flow cytometric detection of endothelial microparticles (EMP): effects of centrifugation and storage alter with the phenotype studied.

Thrombosis research - 1 Apr 2010

van Ierssel Sabrina H, Van Craenenbroeck Emeline M, Conraads Viviane M, Van Tendeloo Viggo F, Vrints Christiaan J, Jorens Philippe G, Hoymans Vicky Y

Abstract excerpt

INTRODUCTION: Endothelial microparticles (EMP) are released into the circulation in case of endothelial disturbance, and are therefore increasingly investigated as a biomarker reflecting disease activity. Numerous pre-analytic methods have been proposed for their flow cytometric enumeration, but standardization is still lacking. In this study we evaluated the influence of centrifugation and storage conditions on...

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PMID: 20117824
DOI: 10.1016/j.thromres.2009.12.019

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