Article
High-reproducible flow cytometric endothelial progenitor cell determination in human peripheral blood as CD34+/CD144+/CD3- lymphocyte sub-population.
Journal of immunological methods - 1 Jun 2008
Redondo Santiago, Hristov Mihail, Gordillo-Moscoso Antonio A, Ruiz Emilio, Weber Christian, Tejerina Teresa
Abstract excerpt
Although determination of circulating endothelial progenitor cell (EPC) in peripheral blood by flow cytometry is an emerging marker for cardiovascular medicine, a common standardized protocol is still not available, due to the low numbers achieved in peripheral blood. In the present paper we describe a novel technique for EPC quantification as CD34+/CD144+/CD3- cells within the lymphocyte gate, which increases...
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