Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

rRNA binding domain of yeast ribosomal protein L25. Identification of its borders and a key leucine residue.

Journal of molecular biology - 20 Mar 1991

Rutgers C A, Rientjes J M, van 't Riet J, Raué H A

Abstract excerpt

We have delineated the region of yeast ribosomal protein L25 responsible for its specific binding to 26 S rRNA by a novel approach using in vitro synthesized, [35S]methionine-labeled fragments as well as point mutants of the L25 protein. The rRNA binding capacity of these mutant polypeptides was tested by incubation with an in vitro transcribed, biotinylated fragment of yeast 26 S rRNA that contains the complete...

Topics

Base Sequence
Binding Sites
Chemical Precipitation
DNA, Fungal
Electrophoresis, Polyacrylamide Gel
Leucine
Molecular Sequence Data
Mutation
RNA, Fungal
RNA, Ribosomal
Ribosomal Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Saccharomyces

Saccharomyces cerevisiae

Source

Identifiers

PMID: 2010915
DOI: 10.1016/0022-2836(91)90719-m

Genes

SDS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2010915-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

rRNA binding domain of yeast ribosomal protein L25. Identification of its borders and a key leucine residue.PMID 2010915Read ↗

Domain III of Saccharomyces cerevisiae 25 S ribosomal RNA: its role in binding of ribosomal protein L25 and 60 S subunit formation.Semantic proximity Read ↗

All three functional domains of the large ribosomal subunit protein L25 are required for both early and late pre-rRNA processing steps in Saccharomyces cerevisiae.Semantic proximity Read ↗

Saccharomyces cerevisiae ribosomal protein L26 is not essential for ribosome assembly and function.Semantic proximity Read ↗

Structure/function analysis of yeast ribosomal protein L2.Semantic proximity Read ↗

Involvement of lysine 270 and lysine 271 of yeast 5S rRNA binding protein in RNA binding and ribosome assembly.Semantic proximity Read ↗

Ribosomal protein L35 is required for 27SB pre-rRNA processing in Saccharomyces cerevisiae.Semantic proximity Read ↗

Mutant forms of Escherichia coli protein L25 unable to bind to 5S rRNA are incorporated efficiently into the ribosome in vivo.Semantic proximity Read ↗

A flexible loop in yeast ribosomal protein L11 coordinates P-site tRNA binding.Semantic proximity Read ↗

Conformational changes induced in the Saccharomyces cerevisiae GTPase-associated rRNA by ribosomal stalk components and a translocation inhibitor.Semantic proximity Read ↗

The central core region of yeast ribosomal protein L11 is important for subunit joining and translational fidelity.Semantic proximity Read ↗

Linker 2 of the eukaryotic pre-ribosomal processing factor Mrd1p is an essential interdomain functionally coupled to upstream RNA Binding Domain 2 (RBD2).Semantic proximity Read ↗

Evolution of the large-subunit ribosomal RNA binding site for protein L23/25.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.