Article
rRNA binding domain of yeast ribosomal protein L25. Identification of its borders and a key leucine residue.
Journal of molecular biology - 20 Mar 1991
Rutgers C A, Rientjes J M, van 't Riet J, Raué H A
Abstract excerpt
We have delineated the region of yeast ribosomal protein L25 responsible for its specific binding to 26 S rRNA by a novel approach using in vitro synthesized, [35S]methionine-labeled fragments as well as point mutants of the L25 protein. The rRNA binding capacity of these mutant polypeptides was tested by incubation with an in vitro transcribed, biotinylated fragment of yeast 26 S rRNA that contains the complete...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
